OSM-HM101 | 价格

OSM-HM101-100μg / 询价

OSM-HM101-500μg / 询价

OSM-HM101-500μgx2 / 询价

Human OSMR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human OSMR Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Glu28-Met740 [Accession | Q99650-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 82.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human OSMR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human OSMR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human OSMR, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.

背景(Background)

OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.

分子别名(Synonyms)

IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 ;IL-31R subunit β; IL-31R-β; OSMR β

文献(References)

(1) Sharanek A, Burban A, Laaper M, Heckel E, Joyal JS, Soleimani VD, Jahani-Asl A. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun. 2020 Aug 17;11(1):4116. doi: 10.1038/s41467-020-17885-z. PMID: 32807793; PMCID: PMC7431428.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.