Mouse OX40 Ligand/TNFSF4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse OX40 Ligand/TNFSF4 Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Ser51-Leu198.[Accession | P43488]

分子量大小(Molecular Weight)

The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per ug by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse OX40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Mouse OX40 Ligand at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 3.59μg/ml determined by ELISA.

背景(Background)

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.

分子别名(Synonyms)

OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34

文献(References)

(1)Al-Shamkhani A , Mallett S , Brown M H , et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282.

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