P40-CM112 | 价格

P40-CM112-100μg / 询价

P40-CM112-500μg / 询价

P40-CM112-500μgx2 / 询价

Cynomolgus IL-12B/p40/NKSF2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ile23-Ser328 [Accession | A0A2K5TM36].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.86 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus IL-12B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus IL-12B is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus IL-12B, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test).

背景(Background)

Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.

分子别名(Synonyms)

IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS

文献(References)

(1) Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M. Interleukin‑12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts. Mol Med Rep. 2016 Apr;13(4):3647-52. doi: 10.3892/mmr.2016.4985. Epub 2016 Mar 7. PMID: 26956410.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.