P40-HM112 | 价格

P40-HM112-100μg / 询价

P40-HM112-500μg / 询价

P40-HM112-500μgx2 / 询价

Human IL-12B/p40/NKSF2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ile23-Ser328 [Accession | P29460].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-12B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human IL-12B, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human IL-12B, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.

SPR Data

Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).

背景(Background)

Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.

分子别名(Synonyms)

IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS

文献(References)

(1) Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M. Interleukin‑12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts. Mol Med Rep. 2016 Apr;13(4):3647-52. doi: 10.3892/mmr.2016.4985. Epub 2016 Mar 7. PMID: 26956410.