货号价格

RKL-HM001-100μg / 询价

RKL-HM001-500μg / 询价

RKL-HM001-500μgx2 / 询价

Human RANKL/TNFSF11/CD254 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag. It contains Gly63-Asp244.[Accession | O14788-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 90% as determined by Tris-Bis PAGE
> 90% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human RANKL on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

SEC-HPLC

The purity of Human RANKL is greater than 90% as determined by SEC-HPLC.

ELISA Data

Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test).

SPR Data

Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).

背景(Background)

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

分子别名(Synonyms)

ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

文献(References)

(1) Nagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61(6):534-42. doi: 10.1159/000371845. Epub 2015 Feb 14. PMID: 25720990.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.