SEM-HM17A | 价格

SEM-HM17A-100μg / 询价

SEM-HM17A-500μg / 询价

SEM-HM17A-500μgx2 / 询价

Human SEMA7A Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln45-Ala648 [Accession | O75326-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 69.5 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human SEMA7A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human SEMA7A is greater than 95% as determined by SEC-HPLC.

背景(Background)

Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.

分子别名(Synonyms)

Semaphorin-7A; Semaphorin-K1; Semaphorin-L; Sema L; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; Sema7A; SEMAK1; SEMAL

文献(References)

(1) Deng J, Xu T, Yang J, Zhang KM, Li Q, Yu XY, Li R, Fu J, Jiang Q, Ma JX, Chen YM. Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats. CNS Neurosci Ther. 2020 Jan;26(1):101-116. doi: 10.1111/cns.13181. Epub 2019 Jun 9. PMID: 31179640; PMCID: PMC6930824.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.