货号价格

SHH-HE001-100μg / 询价

SHH-HE001-500μg / 询价

SHH-HE001-500μgx2 / 询价

Human Sonic Hedgehog (Shh) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag. It contains Cys24-Gly197.[Accession | Q15465]

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.05EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Sonic Hedgehog (Shh) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.

背景(Background)

Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.

分子别名(Synonyms)

SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS

文献(References)

Hosoya A, Shalehin N, Takebe H, Shimo T, Irie K. Sonic Hedgehog Signaling and Tooth Development. Int J Mol Sci. 2020 Feb 26;21(5):1587. doi: 10.3390/ijms21051587. PMID: 32111038; PMCID: PMC7084732.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.