货号价格

SRP-HM40G-100μg / 询价

SRP-HM40G-500μg / 询价

SRP-HM40G-1mg / 询价

Human SIRP Gamma/CD172g Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SIRP Gamma/CD172g Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu29-Pro360.[Accession | Q9P1W8]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human SIRP Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%..

SEC-HPLC

The purity of Human SIRP Gamma is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human SIRP Gamma, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

背景(Background)

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

分子别名(Synonyms)

CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2

文献(References)

(1)Piccio, L. Adhesion of human T cells to antigen-presenting cells through SIRP beta2-CD47 interaction costimulates T-cell proliferation[J]. Blood, 2005, 105(6):2421-2427.

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