货号价格

ST2-HM2L1-100μg / 询价

ST2-HM2L1-500μg / 询价

ST2-HM2L1-500μgx2 / 询价

Human IL-1RL1/ST2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-1RL1/ST2 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys19-Phe328.[Accession | Q01638-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.75 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-1RL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-1RL1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-33, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

背景(Background)

IL1RL1(also known as ST2) is a member of the IL1 receptor family and serves as the receptor for IL-33. The ST2 protein has two isoforms including sST2 and the membrane-bound ST2 receptor, referred to as ST2L. Preclinical studies have demonstrated that binding of IL-33 to ST2L elicits a cardioprotective effect. ST2/IL-33 signaling may play an important role in intestinal disease.

分子别名(Synonyms)

IL1RL1; DER4; ST2; T1; FIT-1; IL33R; ST2L; ST2V; IL-1 R4; IL1R4; Ly84; MGC32623

文献(References)

[1] Griesenauer B, Paczesny S. The ST2/IL-33 Axis in Immune Cells during Inflammatory Diseases. Front Immunol. 2017 Apr 24;8:475.

[2] Vianello E, Dozio E, Tacchini L, Frati L, Corsi Romanelli MM. ST2/IL-33 signaling in cardiac fibrosis. Int J Biochem Cell Biol. 2019 Nov;116:105619.

[3] Dattagupta A, Immaneni S. ST2: Current status. Indian Heart J. 2018 Jul;70 Suppl 1(Suppl 1):S96-S101.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.