TFR-HM401B-100μg / 询价
TFR-HM401B-500μg / 询价
TFR-HM401B-500μgx2 / 询价
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Biotinylated Human Transferrin R/CD71 Protein
Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Cys89-Phe760.[Accession | P02786]
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 80-85 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin R is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-Transferrin R Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 41.4ng/ml determined by ELISA.
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
(1) Jhee JH, Nam BY, Park JT, Kim HW, Chang TI, Kang EW, Lim BJ, Yoo TH, Kang SW, Jeong HJ, Han SH. CD71 mesangial IgA1 receptor and the progression of IgA nephropathy. Transl Res. 2021 Apr;230:34-43. doi: 10.1016/j.trsl.2020.10.007. Epub 2020 Oct 24. PMID: 33122053.
