TG1-CM102 | 价格

TG1-CM102-100μg / 询价

TG1-CM102-500μg / 询价

TG1-CM102-500μgx2 / 询价

Rhesus macaque Latent TGF beta 1/TGFB1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Rhesus macaque Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag at the N-Terminus.
It contains Leu30-Ser390(C33S) [Accession | F7HCV5].

分子量大小(Molecular Weight)

The protein has a predicted MW of 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Rhesus macaque Latent TGF beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 0.74μg/ml determined by ELISA.

背景(Background)

Latent TGF beta 1  cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.

分子别名(Synonyms)

CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1

文献(References)

(1) Robertson I B , Rifkin D B. Regulation of the Bioavailability of TGF-β and TGF-β-Related Proteins[J]. Cold Spring Harbor Perspectives in Biology, 2016, 8(6):a021907.