Human TIGIT Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human TIGIT Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met22-Pro141.[Accession | Q495A1-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18ng/ml determined by ELISA.

Blocking Data

Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml.

SPR Data

Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200).

背景(Background)

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

分子别名(Synonyms)

TIGIT; VSIG9; VSTM3; WUCAM

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

恺佧生物科技(上海)有限公司
电话:400-614-0008
地址:上海市浦江高科技园新骏环路188号8A区四楼
在线留言