货号价格

TNF-HM1R2B-100μg / 询价

TNF-HM1R2B-500μg / 询价

TNF-HM1R2B-500μgx2 / 询价

Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.
It contains Leu23-Asp257 [Accession | P20333-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human TNFR2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human TNFR2, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.

背景(Background)

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

分子别名(Synonyms)

CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B

文献(References)

(1)Vanamee, éva S, Faustman D L. TNFR2: A Novel Target for Cancer Immunotherapy[J]. Trends in Molecular Medicine, 2017:S1471491417301697.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.