TNF-HM212-100μg / 询价
TNF-HM212-500μg / 询价
TNF-HM212-500μgx2 / 询价
Human TNFSF12/TWEAK Protein
Recombinant Human TNFSF12/TWEAK Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser43-His249 [Accession | O43508-1].
The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA (QC Test).
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
Wang M, Xie Z, Xu J, Feng Z. TWEAK/Fn14 axis in respiratory diseases. Clin Chim Acta. 2020 Oct;509:139-148. doi: 10.1016/j.cca.2020.06.007. Epub 2020 Jun 8. PMID: 32526219.
