VEG-HM010 | 价格

VEG-HM010-100μg / 询价

VEG-HM010-500μg / 询价

VEG-HM010-500μgx2 / 询价

Human VEGF110 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.
It contains Ala27-Arg136 [Accession | P15692-9].

分子量大小(Molecular Weight)

The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human VEGF110, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16μg/ml determined by ELISA (QC Test).

背景(Background)

Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified.

分子别名(Synonyms)

VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1

文献(References)

Jing J, Zhong X, Liu BC, Lyu LL. [Research progress of vascular endothelial growth factor-A and its isoforms in kidney disease]. Sheng Li Xue Bao. 2022 Feb 25;74(1):59-66. Chinese. PMID: 35199126.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.