货号价格

AFP-CM101-100μg / 询价

AFP-CM101-500μg / 询价

AFP-CM101-500μgx2 / 询价

Cynomolgus AFP Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus AFP Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Arg19-Val609 [Accession | A0A2K5U7U0].

分子量大小(Molecular Weight)

The protein has a predicted MW of 67.53 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus AFP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus AFP is greater than 95% as determined by SEC-HPLC.

背景(Background)

Alpha-fetoprotein is a shuttle protein that delivers nutrients through receptor-mediated endocytosis to embryotic cells. In adults, alpha-fetoprotein can shuttle drugs into alpha-fetoprotein receptor-positive myeloid-derived suppressor, regenerating and also cancer cells. Drugs with high-binding affinity to alpha-fetoprotein can activate or deplete targeted cells. Myeloid-derived suppressor cells activation leads to immune suppression that can be used for treating autoimmune diseases.

分子别名(Synonyms)

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; HPAFP; AFPD; FETA

文献(References)

(1) Pak VN. The use of alpha-fetoprotein for the treatment of autoimmune diseases and cancer. Ther Deliv. 2018 Jan;9(1):37-46. doi: 10.4155/tde-2017-0073. PMID: 29216804.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.