Human APOE4/Apolipoprotein E Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human APOE4/Apolipoprotein E Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Lys19-His317.[Accession | AAB59397.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS, 2mM DTT, 5mM CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human APOE4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SPR Data

Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay (Biacore T200).

背景(Background)

Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

分子别名(Synonyms)

Apolipoprotein E; Apo-E; APOE; apolipo E; APOE4

文献(References)

(1) Zhao N, Liu CC, Qiao W, Bu G. Apolipoprotein E, Receptors, and Modulation of Alzheimer's Disease. Biol Psychiatry. 2018 Feb 15;83(4):347-357. doi: 10.1016/j.biopsych.2017.03.003. Epub 2017 Mar 14. PMID: 28434655; PMCID: PMC5599322.

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