APR-HM410B-100μg / 询价
APR-HM410B-500μg / 询价
APR-HM410B-500μgx2 / 询价
Biotinylated Human APRIL/TNFSF13 Trimer Protein
Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag Tag at the N-Terminus.It contains Lys112-Leu250 [Accession | O75888-1].
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Biotinylated Human APRIL is greater than 95% as determined by SEC-HPLC.
Immobilized Human BCMA, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
