Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from Expi293 with monomeric hFc tag at the N-terminal. It contains Arg71-Glu254.[Accession | P41273]

分子量大小(Molecular Weight)

The protein has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human 4-1BB Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer) , hFc Tag with the EC50 of 61ng/ml determined by ELISA.

背景(Background)

The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

分子别名(Synonyms)

41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9

文献(References)

(1)Vinay D S , Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.

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