Biotinylated Human BCMA/TNFRSF17 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Met1-Ala54 [Accession | Q02223-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 8.9 kDa. Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Biotinylated Human BCMA, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human APRIL (Trimer), His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 38.2ng/ml determined by ELISA.

ELISA Data

Immobilized Anti-BCMA Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.

背景(Background)

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

分子别名(Synonyms)

CD269; TNFRSF17; BCMA; BCM; TNFRSF13A

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.