CAM-HM406B | 价格

CAM-HM406B-100μg / 询价

CAM-HM406B-500μg / 询价

CAM-HM406B-500μgx2 / 询价

Biotinylated Human CEACAM-6/CD66c Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CEACAM-6/CD66c Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Lys35-Gly320 [Accession | P40199].

分子量大小(Molecular Weight)

The protein has a predicted MW of 34.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human CEACAM-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human CEACAM-6 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Biotinylated Human CEACAM6, His-Avi Tag with an affinity constant of 3.81 μM as determined in SPR assay (Biacore T200).

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) belongs to the human carcino-embryonic antigen (CEA) family. Numerous lines of studies have indicated that altered expression of CEACAM6 may have a role in carcinogenesis and development.

分子别名(Synonyms)

CEACAM6; CD66c; CEAL; NCA; CEA

文献(References)

(1)Sharma N, Srivastava S, Kern F, et al. CEACAM 6, a novel marker for the diagnosis of Barrett's esophagus. Dis Esophagus. 2017;30(7):1-5. doi:10.1093/dote/dox026

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.