CEA-HM108 | 价格

CEA-HM108-100μg / 询价

CEA-HM108-500μg / 询价

CEA-HM108-500μgx2 / 询价

Human CEACAM-8/CD66b Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln35-Ser319 [Accession | NP_001807.2].

分子量大小(Molecular Weight)

The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CEACAM-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 4.59 μM as determined in SPR assay (Biacore T200).

背景(Background)

Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.

分子别名(Synonyms)

CD66b; CEACAM8; CGM6; CD67; NCA-95

文献(References)

(1) Yoon J, Terada A, Kita H. CD66b regulates adhesion and activation of human eosinophils. J Immunol. 2007 Dec 15;179(12):8454-62. doi: 10.4049/jimmunol.179.12.8454. PMID: 18056392.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.