SARS-COV-2 Spike S Trimer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant SARS-COV-2 Spike S Trimer Protein is expressed from HEK293 with monomeric hFc tag at the C-Terminus. It contains Val16-Glu1188.[Accession | QHD43416.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 158.3 kDa. Due to glycosylation, the protein migrates to 230-250 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

SARS-COV-2 Spike S (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS-COV-2 Spike S (Trimer) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22μg/ml determined by ELISA.

背景(Background)

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

分子别名(Synonyms)

S protein; Spike glycoprotein; S glycoprotein; COVID-19

文献(References)

(1)Parsons, Lisa, Bouwman, Kim M, Azurmendi, Hugo F. Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding[J]. Journal of Biological Chemistry.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.