DKK-HM201-100μg / 询价
DKK-HM201-500μg / 询价
DKK-HM201-500μgx2 / 询价
Human DKK1 Protein
Recombinant Human DKK1 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Thr32-His266 [Accession | O94907].
The protein has a predicted MW of 52.54 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DKK1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human DKK1, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69μg/ml determined by ELISA.
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Dickkopf-1; Dkk1; SK
(1)Zhang W , Drake M T. Potential Role for Therapies Targeting DKK1, LRP5, and Serotonin in the Treatment of Osteoporosis[J]. Current Osteoporosis Reports, 2012, 10(1):93-100.
