Human LRP-5 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.
It contains Glu644-Gln1263 [Accession | O75197-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human LRP-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human Wnt Surrogate-Fc Fusion, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.73μg/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63μg/ml determined by ELISA.

背景(Background)

A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.

分子别名(Synonyms)

LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2

文献(References)

(1)Williams BO. LRP5: From bedside to bench to bone. Bone. 2017 Sep;102:26-30. doi: 10.1016/j.bone.2017.03.044. Epub 2017 Mar 21. PMID: 28341377.