EFN-HM2A3 | 价格

EFN-HM2A3-100μg / 询价

EFN-HM2A3-500μg / 询价

EFN-HM2A3-500μgx2 / 询价

Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln23-Ser213 [Accession | P52797-1(Human) / A0A2K5VZR7(Cynomolgus) / NP_001252612.1(Rhesus macaque)].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human/Cynomolgus/Rhesus macaque Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human/Cynomolgus/Rhesus macaque Ephrin-A3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human EPHA10, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).

背景(Background)

Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.

分子别名(Synonyms)

Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3

文献(References)

(1) Duffy SL, Steiner KA, Tam PP, Boyd AW. Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Gene Expr Patterns. 2006 Oct;6(7):719-23. doi: 10.1016/j.modgep.2005.12.007. Epub 2006 Feb 8. PMID: 16466970.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.