FSF-MM215 | 价格

FSF-MM215-100μg / 询价

FSF-MM215-500μg / 询价

FSF-MM215-500μgx2 / 询价

Mouse TNFSF15 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with hFc tag at the N-Terminus.
It contains Ala61-Leu252 [Accession | Q5UBV8].

分子量大小(Molecular Weight)

The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse TNFSF15 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse DR3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFSF15, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test)..

SPR Data

Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).

背景(Background)

TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.

分子别名(Synonyms)

TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A

文献(References)

(1) Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019 Mar 27;10:583. doi: 10.3389/fimmu.2019.00583. PMID: 30972074; PMCID: PMC6445966.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.