Human GDF15 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.
It contains Ala197-Ile308 [Accession | Q99988-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

ELISA Data

Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).

Blocking Data

Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

SPR Data

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

背景(Background)

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

分子别名(Synonyms)

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β

文献(References)

(1)Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.