GFL-MM401-100μg / 询价
GFL-MM401-500μg / 询价
GFL-MM401-500μgx2 / 询价
Mouse GFRAL/GFR alpha-like Protein
Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu350 [Accession | Q6SJE0-1].
The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse GFRAL, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
GFR alpha-like; GFRAL; GRAL; C6orf144
(1) Yang L, Chang CC, Sun Z, et al. GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand. Nat Med. 2017;23(10):1158-1166. doi:10.1038/nm.4394
