货号价格

GSF-HE001-100μg / 询价

GSF-HE001-500μg / 询价

GSF-HE001-500μgx2 / 询价

Human GM-CSF Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GM-CSF Protein is expressed from E.coli without tag.
It contains Ala18-Glu144 [Accession | P04141].

分子量大小(Molecular Weight)

The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GM-CSF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).

SPR Data

Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).

Cell Based Assay

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).

背景(Background)

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

分子别名(Synonyms)

CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897

文献(References)

(1) Arellano M , Lonial S. Clinical uses of GM-CSF, a critical appraisal and update[J]. Targets & therapy, 2008, 2(1):13-27.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.