Mouse IL-1R2/IL-1 RII/CD121b Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Phe14-Glu355 [Accession | P27931].

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse IL-1R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse IL-1R2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse IL-1R2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2μg/ml determined by ELISA (QC Test).

背景(Background)

Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity.

分子别名(Synonyms)

IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β

文献(References)

(1)Zhang L, Qiang J, Yang X, et al. IL1R2 Blockade Suppresses Breast Tumorigenesis and Progression by Impairing USP15-Dependent BMI1 Stability. Adv Sci (Weinh). 2019;7(1):1901728. Published 2019 Nov 13. doi:10.1002/advs.201901728

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.