IL1-ME40BB-100μg / 询价
IL1-ME40BB-500μg / 询价
IL1-ME40BB-500μgx2 / 询价
Biotinylated Mouse IL-1 Beta/IL-1F2 Protein
Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Val118-Ser269 [Accession | P10749].
The protein has a predicted MW of 20.30 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test).
Immobilized Mouse IL-1R2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2μg/ml determined by ELISA.
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β
(1) Bent R, Moll L, Grabbe S, Bros M. Interleukin-1 Beta-A Friend or Foe in Malignancies? Int J Mol Sci. 2018 Jul 24;19(8):2155. doi: 10.3390/ijms19082155. PMID: 30042333; PMCID: PMC6121377.
