IL2-HM125-100μg / 询价
IL2-HM125-500μg / 询价
IL2-HM125-500μgx2 / 询价
Human IL-25/IL-17E Protein
Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177 [Accession | Q9H293-1].
The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-25, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46μg/ml determined by ELISA (QC Test).
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
(1) Xu M, Dong C. IL-25 in allergic inflammation. Immunol Rev. 2017 Jul;278(1):185-191. doi: 10.1111/imr.12558. PMID: 28658555.
