IL3-HE401-100μg / 询价
IL3-HE401-500μg / 询价
IL3-HE401-500μgx2 / 询价
Human IL-3 Protein
Recombinant Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152 [Accession | P08700].
The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46μg/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
(1)Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.
