IL3-HE401B | 价格

IL3-HE401B-100μg / 询价

IL3-HE401B-500μg / 询价

IL3-HE401B-500μgx2 / 询价

Biotinylated Human IL-3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.
It contains Ala20-Phe152 [Accession | P08700].

分子量大小(Molecular Weight)

The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human IL-3, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test).

背景(Background)

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

分子别名(Synonyms)

IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

文献(References)

(1)Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.