IL8-MM1BP | 价格

IL8-MM1BP-100μg / 询价

IL8-MM1BP-500μg / 询价

IL8-MM1BP-500μgx2 / 询价

Mouse IL-18BP Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Thr29-Ala193 [Accession | Q9Z0M9].

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Mouse IL-18BP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).

SPR Data

Mouse IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.86 nM as determined in SPR assay (Biacore T200).

背景(Background)

Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.

分子别名(Synonyms)

IL-18BP; Igifbp

文献(References)

(1) Zhou T, Damsky W, Weizman OE, McGeary MK, Hartmann KP, Rosen CE, Fischer S, Jackson R, Flavell RA, Wang J, Sanmamed MF, Bosenberg MW, Ring AM. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature. 2020 Jul;583(7817):609-614. doi: 10.1038/s41586-020-2422-6. Epub 2020 Jun 24. PMID: 32581358; PMCID: PMC7381364.