IL8-MM2BP | 价格

IL8-MM2BP-100μg / 询价

IL8-MM2BP-500μg / 询价

IL8-MM2BP-500μgx2 / 询价

Mouse IL-18BP Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse IL-18BP Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Thr29-Ala193 [Accession | Q9Z0M9].

分子量大小(Molecular Weight)

The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse IL-18BP is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse IL-18BP, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 8.2ng/ml determined by ELISA.

背景(Background)

Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.

分子别名(Synonyms)

IL-18BP; Igifbp

文献(References)

(1) Zhou T, Damsky W, Weizman OE, McGeary MK, Hartmann KP, Rosen CE, Fischer S, Jackson R, Flavell RA, Wang J, Sanmamed MF, Bosenberg MW, Ring AM. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature. 2020 Jul;583(7817):609-614. doi: 10.1038/s41586-020-2422-6. Epub 2020 Jun 24. PMID: 32581358; PMCID: PMC7381364.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.