ITG-MM1V8-100μg / 询价
ITG-MM1V8-500μg / 询价
ITG-MM1V8-500μgx2 / 询价
Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein
Recombinant Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe31-Val988(ITGAV) acidic tail & Gly22-Ser679(ITGB8) basic tail [Accession | P43406(ITGAV)&Q0VBD0(ITGB8)].
The protein has a predicted MW of 111.6(ITGAV) & 76.6(ITGB8) kDa. Due to glycosylation, the protein migrates to 80-140 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse ITGAV&ITGB8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse ITGAV&ITGB8 is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse ITGAV&ITGB8, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.97μg/ml determined by ELISA.
Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.
αVβ8; Integrin alpha V beta 8;ITGAV&ITGB8;Integrin alpha V β 8
(1) Arnold TD, Ferrero GM, Qiu H, Phan IT, Akhurst RJ, Huang EJ, Reichardt LF. Defective retinal vascular endothelial cell development as a consequence of impaired integrin αVβ8-mediated activation of transforming growth factor-β. J Neurosci. 2012 Jan 25;32(4):1197-206. doi: 10.1523/JNEUROSCI.5648-11.2012. Erratum in: J Neurosci. 2012 Apr 11;32(15):5351. PMID: 22279205; PMCID: PMC3578416.
