恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Mouse Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus It contains Phe53-Pro994 [Accession \| Q99N32-1].
分子量大小（Molecular Weight）	The protein has a predicted MW of 109.91 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Bis-Tris PAGE result.
纯度（Purity）	>90% as determined by Bis-Tris PAGE; >90% as determined by HPLC
内毒素（Endotoxin）	Less than 1 EU per μg by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储（Storage）	-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

表达区间及表达系统（Source）

Recombinant Mouse Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus
It contains Phe53-Pro994 [Accession | Q99N32-1].

分子量大小（Molecular Weight）

The protein has a predicted MW of 109.91 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Bis-Tris PAGE result.

纯度（Purity）

>90% as determined by Bis-Tris PAGE; >90% as determined by HPLC

内毒素（Endotoxin）

Less than 1 EU per μg by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储（Storage）

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.