KLK-HM12P | 价格

KLK-HM12P-100μg / 询价

KLK-HM12P-500μg / 询价

KLK-HM12P-500μgx2 / 询价

Human Kallikrein 2/KLK2 Protein (pro form)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Kallikrein 2/KLK2 Protein (pro form) is expressed from HEK293 with His tag at the C-terminus. The protein needs to be activated by Trypsin for an activated form.
It contains Pro19-Pro261 with modified propeptide [Accession | P20151-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.43 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose (pH 8.0).

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human Kallikrein 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human Kallikrein 2 is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human Kallikrein 2 (pro form), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. (QC Test)
Bioactivity Data
Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >150 pmol/min/µg. (QC Test)
背景(Background)

Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.

分子别名(Synonyms)

KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2

文献(References)

Jairajpuri DS, Hussain A, Alajmi MF, Mohammad T, Shamsi A, Hassan MI. Structure-based identification of bioactive phytochemicals targeting kallikrein-related peptidase 2 for prostate cancer therapy. Front Chem. 2025 Mar 26;13:1553987. doi: 10.3389/fchem.2025.1553987. PMID: 40207180; PMCID: PMC11979280.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.