货号价格

MCP-HM401B-100μg / 询价

MCP-HM401B-500μg / 询价

MCP-HM401B-500μgx2 / 询价

Biotinylated Human MCP-1/CCL2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human MCP-1/CCL2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln24-Thr99.[Accession | P13500]

分子量大小(Molecular Weight)

The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human MCP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human MCP-1 is greater than 95% as determined by SEC-HPLC.

背景(Background)

Monocyte chemotactic protein-1 (MCP-1) (also referred to as chemokine (C-C motif) ligand 2 (CCL2) is expressed by mainly inflammatory cells and endothelial cells. MCP-1 has been reported to play an important role in the pathogenesis of atherosclerosis and considerable evidence supports that the monocyte containing MCPs and macrophage influences the growth of other cell types within the atherosclerotic lesion. This review will focus on the general structure features of MCP-1 and its role in atherosclerosis.

分子别名(Synonyms)

C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; HSMCR30; MCP1; SCYA2; SMC-CF; GDCF-2

文献(References)

(1)Lin J, Kakkar V, Lu X. Impact of MCP-1 in atherosclerosis. Curr Pharm Des. 2014;20(28):4580-4588. doi:10.2174/1381612820666140522115801

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.