货号价格

PLA-HM102-100μg / 询价

PLA-HM102-500μg / 询价

PLA-HM102-500μgx2 / 询价

Human PLAU/uPA Protein (pro form)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human PLAU/uPA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus.
It contains Ser21-Leu431 [Accession | P00749-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human PLAU (pro form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

背景(Background)

Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

分子别名(Synonyms)

PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD

文献(References)

(1) Chen G, Sun J, Xie M, Yu S, Tang Q, Chen L. PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma. Front Genet. 2021 May 20;12:651882. doi: 10.3389/fgene.2021.651882. PMID: 34093649; PMCID: PMC8173099.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.