Cynomolgus uPAR/PLAUR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus uPAR/PLAUR Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Leu23-Gly305 [Accession | Q9GK78].

分子量大小(Molecular Weight)

The protein has a predicted MW of 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus uPAR, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).

SPR Data

Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).

背景(Background)

The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

分子别名(Synonyms)

U-PAR; uPAR; CD87; PLAUR; MO3; UPAR

文献(References)

(1) Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID: 9122717.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.