恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Biotinylated Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155) [Accession \| P00749-1].
分子量大小（Molecular Weight）	The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Bis-Tris PAGE result.
纯度（Purity）	> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素（Endotoxin）	Less than 1EU per ug by the LAL method.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储（Storage）	-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

表达区间及表达系统（Source）

Recombinant Biotinylated Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155) [Accession | P00749-1].

分子量大小（Molecular Weight）

The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Bis-Tris PAGE result.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储（Storage）

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.