货号价格

PLA-MM401B-100μg / 询价

PLA-MM401B-500μg / 询价

PLA-MM401B-500μgx2 / 询价

Biotinylated Mouse PLAU/uPA Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Mouse PLAU/uPA Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.
It contains Gly21-Phe433 [Accession | P06869].

分子量大小(Molecular Weight)

The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Mouse PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Mouse PLAU is greater than 95% as determined by SEC-HPLC.

SPR Data

Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 0.19 μM as determined in SPR assay (Biacore T200).

背景(Background)

Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

分子别名(Synonyms)

PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD

文献(References)

(1) Chen G, Sun J, Xie M, Yu S, Tang Q, Chen L. PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma. Front Genet. 2021 May 20;12:651882. doi: 10.3389/fgene.2021.651882. PMID: 34093649; PMCID: PMC8173099.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.