RNK-MM211-100μg / 询价
RNK-MM211-500μg / 询价
RNK-MM211-500μgx2 / 询价
Mouse TNFRSF11A/Rank Protein
Recombinant Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Val31-Ser214.[Accession | O35305]
The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse TNFRSF11A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse RANKL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.
ODFR; Receptor activator of NF-KB; NF-KB; NFKB; CD265; TNFRSF11A; Rank; FEO; LOH18CR1; OPTB7; OSTS; PDB2; TRANCER;
von dem Knesebeck A, Felsberg J, Waha A, Hartmann W, Scheffler B, Glas M, Hammes J, Mikeska T, Yan PS, Endl E, Simon M, Reifenberger G, Pietsch T, Waha A. RANK (TNFRSF11A) is epigenetically inactivated and induces apoptosis in gliomas. Neoplasia. 2012 Jun;14(6):526-34. doi: 10.1596/neo.12360. PMID: 22787434; PMCID: PMC3394195.
