SED-HM107 | 价格

SED-HM107-100μg / 询价

SED-HM107-500μg / 询价

SED-HM107-500μgx2 / 询价

Human SETD7 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SETD7 Protein is expressed from E.coli with His tag at the N-Terminus.
It contains Met1-Lys366 [Accession | NP_085151.1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.68 kDa. The protein migrates to 47-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human SETD7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human SETD7 is greater than 95% as determined by SEC-HPLC.

背景(Background)

SETD7 is a methyltransferase that specifically catalyzes the monomethylation of lysine 4 on histone H3. A variety of studies has revealed the role of SETD7 in posttranslational modifications of non-histone proteins. Aberrant expression of SETD7 has been associated with various diseases, including cancer. As a prognostic marker of breast cancer and a novel antioxidant promoter under oxidative stress in breast cancer, SETD7 is considered a good target for the development of new epigenetic drugs.

分子别名(Synonyms)

KIAA1717; KMT7; SET7; SET9; SET7/9

文献(References)

Lenstra DC, Damen E, Leenders RGG, Blaauw RH, Rutjes FPJT, Wegert A, Mecinović J. Structure-Activity Relationship Studies on (R)-PFI-2 Analogues as Inhibitors of Histone Lysine Methyltransferase SETD7. ChemMedChem. 2018 Jul 18;13(14):1405-1413. doi: 10.1002/cmdc.201800242. Epub 2018 Jun 25. PMID: 29869845.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.