SOT-HM201 | 价格

SOT-HM201-100μg / 询价

SOT-HM201-500μg / 询价

SOT-HM201-500μgx2 / 询价

Human SOST/Sclerostin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.
It contains Gln24-Tyr213 [Accession | Q9BQB4-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 47.05 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
ELISA Data
Immobilized Human SOST, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA. (QC Test)
BLI Data
Loaded Human SOST, hFc Tag on Anti-Human lgG Fc Gen Il (HFc Il) -Biosensor can bind Human LRP-6, mFc Tag (Cat. LRP-HM316) with an affinity constant of 3.95 nM as determined in BLI assay.
背景(Background)

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

分子别名(Synonyms)

sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH

文献(References)

(1)Claire M N , Patton D , Hayes J S. Sclerostin Antibody Therapy for the Treatment of Osteoporosis: Clinical Prospects and Challenges[J]. Journal of Osteoporosis, 2016, 2016:1-22.