Biotinylated Human SIRP alpha V5 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human SIRP alpha V5 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu31-Arg370(S42F).[Accession | P78324 variant 5]

分子量大小(Molecular Weight)

The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human SIRP alpha V5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human SIRP alpha V5 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.1μg/ml determined by ELISA.

背景(Background)

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

分子别名(Synonyms)

CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA

文献(References)

(1)Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.

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