Mouse TIGIT Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse TIGIT Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gly26-Thr143.[Accession | NP_001139797.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse TIGIT is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse CD155, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.23μg/ml determined by ELISA.

背景(Background)

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

分子别名(Synonyms)

TIGIT; VSIG9; VSTM3; WUCAM

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

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